Improvements in creatinine methodology: a critical assessment

Eur J Clin Chem Clin Biochem. 1994 Jul;32(7):529-37. doi: 10.1515/cclm.1994.32.7.529.

Abstract

A large evaluation study, analytical as well as clinical, was performed on four published improvements for the determination of creatinine in serum. Two of the methods were based on the Jaffé reaction, the other two were enzymatic methods. Analytically, all four methods showed a similar performance. In the analysis of numerous specimens from advanced care departments, however, the methods performed differently. The two enzymatic procedures scored better than the two Jaffé methods, when compared with an HPLC-based reference method. However, even the best methods produced outliers and a larger scatter than that obtained with "normal" specimens.

MeSH terms

  • Bilirubin / blood
  • Chromatography, High Pressure Liquid
  • Creatinine / blood*
  • Critical Care
  • False Positive Reactions
  • Humans
  • Jaundice / blood
  • Organ Transplantation
  • Reference Values
  • Reproducibility of Results

Substances

  • Creatinine
  • Bilirubin